Hermann H. Hess made significant contributions to the understanding of acetylcholine receptor kinetics through various sophisticated techniques. Here are some of the techniques he used:

1. Patch-Clamp Electrophysiology: Hess utilized patch-clamp recordings to measure ionic currents through individual ion channels, allowing detailed analysis of the receptor kinetics at the single-channel level. This method helped in understanding the gating properties and conductance states of acetylcholine receptors.

2. Single-Channel Recording: By focusing on single-channel events, Hess could study the temporal patterns of channel opening and closing, providing insights into the kinetics of receptor activation and desensitization.

3. Rapid Perfusion Techniques: To study the kinetics of acetylcholine receptor activation, Hess employed rapid application and removal of agonists, which allowed him to observe the receptor responses in real-time. This approach was crucial for understanding fast neurotransmission events.

4. Computer Simulations and Modelling: Hess also used computational models to simulate receptor kinetics, helping to interpret electrophysiological data and predict receptor behavior under different experimental conditions.

These techniques, combined with innovative experimental designs, allowed Hess to make significant strides in the field of neurobiology and pharmacology. For more detailed insights, you may explore publications that detail his research methodologies.