Reference

Wice and Gordon used a strategy for isolating cDNAs encoding proteins that affect human intestinal epithelial cell growth and differentiation. They characterized a novel gut-specific N-myristoylated annexin through this approach. The strategy involved identifying sequences that are absent in proliferating cells and present in differentiated cells, allowing them to isolate cDNA clones that represent proteins involved in cell differentiation. This method was crucial for understanding the molecular mechanisms underlying cell differentiation in the human intestinal epithelium. For more details, you can refer to their publication: A strategy for isolation of cDNAs encoding proteins affecting human intestinal epithelial cell growth and differentiation.